Mesocotyl Elongation of Weedy Rice and Its Relationship with Grain Amylase Activities and Soluble Sugar Contents

doi:10.3969/j.issn.1001-7216.2014.01.014

Abstract

Abstract: The dynamic process of mesocotyl elongation of weedy rice was observed in a germination test in a constant temperature incubator in darkness. The changes in amylase activities in embryos and soluble sugar contents in the process of mesocotyl elongation and their relationship were analyzed. The results showed that during the first three days after germination in darkness, the mesocotyl elongation rate was very slow and accelerated four and five days after germination. The mesocotyl elongation gradually stopped seven days after germination. The mesocotyl of the weedy rice was significantly longer than that of Liaojing 294. In the germination process of weedy rice, soluble sugar contents followed an increasing trend, together with αamylase activity and βamylase activity. Mesocotyl elongation length was significantly correlated with the αamylase activity, βamylase activity and soluble sugar contents. The enhanced amylase activities in the beginning of weedy rice germination promoted starch degradation, supplying material and energy for mesocotyl elengation.

Key words: weedy rice, mesocotyl, amylase activity, soluble sugar

摘要： 采用恒温培养箱，在黑暗条件下进行发芽试验，观察杂草稻中胚轴伸长的动态过程，分析杂草稻中胚轴伸长过程中籽粒淀粉酶活性和可溶性糖含量的变化及相互关系。结果表明，黑暗条件下发芽后1 d到3 d，杂草稻中胚轴伸长非常慢，发芽后4 d到5 d中胚轴伸长加快，发芽后7天中胚轴伸长逐渐停止，其最终长度显著长于辽粳294。杂草稻发芽过程中，籽粒α淀粉酶活性、β淀粉酶活性和可溶性糖含量均呈逐渐上升趋势。可溶性糖含量与α淀粉酶活性、β淀粉酶活性均呈极显著正相关。中胚轴伸长长度与籽粒α淀粉酶活性、β淀粉酶活性、可溶性糖含量也均呈极显著正相关。杂草稻发芽初期淀粉酶活性增强，促进了籽粒中淀粉水解为可溶性糖，为中胚轴伸长生长提供了物质和能量基础。

关键词: 杂草稻, 中胚轴, 淀粉酶活性, 可溶性糖

CLC Number:

MA Dianrong*, KONG Dexiu， LIU Xiaoliang, GAO Qi， DING Guohua, ZHAO Minghui， TANG Liang， XU Zhengjin, CHEN Wenfu*. Mesocotyl Elongation of Weedy Rice and Its Relationship with Grain Amylase Activities and Soluble Sugar Contents[J]. Chinese Journal of Rice Science, 2014, 28(1): 97-102.

马殿荣*,孔德秀,刘晓亮,高齐,丁国华,赵明辉,唐亮,徐正进,陈温福*. 杂草稻中胚轴伸长动态及其与籽粒淀粉酶活性和可溶性糖含量的关系[J]. 中国水稻科学, 2014, 28(1): 97-102.

References

\[1\]Zhang Y P, Zhu D F, Xing H, et al. Development and transition of rice planting in China. Agric Sci & Technol, 2012, 13(6): 12701276.

\[2\]吴文革, 陈烨，钱银飞, 等.水稻直播栽培的发展概况与研究进展. 中国农业科技导报, 2006, 8(4): 3236.

\[3\]凌启鸿. 关于水稻轻简栽培问题的探讨. 中国稻米， 1997（5）：39.

\[4\]张光恒，林建荣，吴明国，等. 水稻出苗顶土动力源研究. 中国水稻科学， 2005， 19（1）： 5962.

\[5\]颜启传. 种子学. 北京：中国农业出版社， 2001： 110117.

\[6\]Hoshikawa K. The Growing Rice Plant: An Anatomical Morograph. Japan,Tokyo: Nobunkyo, 1989： 236242.

\[7\]Dilday R H, Mgonga M A, Wells B R. Plant height vs. mesocotyl and elongation in rice: Linkage or pleioutropism. Crop Sci, 1990, 30: 815818.

\[8\]Redona E D, Mackill D J. Mapping quantitative trait loci for seeding vigor in rice using RFLP. Theor Appl Genet, 1996, 92:395402.

\[9\]周德超. 再谈禾谷类种子萌发过程中胚轴的伸长. 生物学通报，1991（9）：10.

\[10\]Redon E D, Mackill D J. Mapping quantitative trait loci for seeding vigor in rice using RFLP. Theor Appl Genet，1996,92:395402.

\[11\]曹立勇，朱军，颜启传，等. 水稻籼粳交DH群体幼苗中胚轴长度的QTLs定位和上位性分析. 中国水稻科学， 2002， 16（3）：221224.

\[12\]林建荣，张光恒，吴明国，等. 水稻中胚轴伸长特性的遗传分析.作物学报， 2006， 32（2）： 249252.

\[13\]马殿荣,王楠,王莹，等.中国北方杂草稻深覆土条件下出苗动力源分析. 中国水稻科学, 2008, 22(2): 215218.

\[14\]Takahashi N. Adaptive importance of mesocotyl and coleoptile growth in rice under different moisture regimes. Aust J Planta Physiol, 1978（5）： 511517.

\[15\]Turner T F, Chen C C， Bollich C N. Coleptile and mesocotyl length in semidwarf rice seedlings. Crop Sci, 1982，22：4346.

\[16\]Faustino M, Lillana C， Leonardo S. Enviromental effects on R()pantoyllactoneβDglucopyranoside accumulation in rice seedlings. Plant physiol Biochem,1997， 35(12):933938.

\[17\]Taeg S N, Byun W L. Effect of seedsoaked GA3 and inorganic salts on mesocotyl and coleoptile elongation in rice. Kor Crop Sci, 2000, 45(1):5054.

\[18\]Azuma T, Hatanaka T, Uchida N, et al. Interactions between abscisic acid, ethylene and gibberellin in internodal elongation in floating rice: The promotive effect of abscisic acid at low humidity. Plant Growth Regul, 2003, 41:105109.

\[19\]Watanabe H, Takahashi K. Effects of abscisic acid and its related compounds on rice seedling growth. Plant Growth Regul， 1999, 28(1): 58.

\[20\]Watanabe H， Takahashi K. Effects of plant growth regulators on the appearance of MC type rice seedlings. Jpn J Crop Sci， 1997,66(2): 318324.

\[21\]Watanabe H， Takahashi K. Effect of abscisic acid, fusicoccin and potassium on growth and morphogenesis of leaves and internode in darkgrown rice seedlings. Plant Growth Regul， 1997， 21: 109114.

\[22\]Watanabe H, Takahashi K， Masahiko S. Morphological and anatomical effects of abscisic acid (ABA) and fluridone (FLU) on the growth of rice mesocotyls. Plant Growth Regul, 2001,34(3): 273275.

\[23\]曹立勇，袁守江，周海鹏，等. 外源激素对水稻中胚轴伸长的影响. 作物学报， 2005， 31（8）： 10981100.

\[24\]郝建军，刘延吉. 植物生理学试验技术. 沈阳：辽宁科学技术出版社， 2001.

\[25\]张志良. 植物生理学实验指导. 北京：高等教育出版社， 1990.

\[26\]角田重三郎，著, 闵绍楷，译. 稻的生物学. 北京: 农业出版社,1989: 121.

\[27\]田中孝庆，著, 朱庆森，译. 水稻的基础生理和生态. 上海: 科技技术出版社, 1987: 9293.

\[28\]王莹，马殿荣，陈温福. 北方杂草稻中胚轴伸长特性的初步研究. 中国稻米， 2008（3）： 4750.

\[29\]Brown H T, Morris G H. Researches on the germination of some of the Gramineae. J Chem Soc, 1980, 57: 458528.

\[30\]陆定志, 施天生, 陈龙飞. 杂交水稻及其亲本三系种子萌发过程中淀粉酶活性与胚乳物质消长的关系. 杂交水稻, 1987(3): 2125.

\[31\]周宏伟. 水稻籽粒在萌发过程中胚乳消耗和淀粉体形态的变化\[学位论文\]. 扬州：扬州大学， 2006: 16.

\[32\]Okamoto K, Akazawa T. Enzymic mechanism of starch breakdown in germinating rice seeds： Amylase formation in the epithelium. Plant Physiol, 1979, 63: 336340.

\[33\]Gomez C A, Zentella R, Walker S M K, et al. Gibberellin/abscisic acid antagonism in barley aleurone cells： Site of action of the protein kinase PKABA1 in relation to gibberellin signaling molecules. Plant Cell, 2001， 13: 667679.

\[34\]陈爱国，陈进红. 胚芽鞘的伸长机理和生理生态响应. 山东农业大学学报：自然科学版， 2002， 33(4)： 438441.

\[35\]赵玉锦, 王台. 水稻种子萌发过程中α淀粉酶与萌发速率关系的分析. 植物学通报, 2001, 18(2): 226230.

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